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tris-buffered saline  (Millipore)


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    Structured Review

    Millipore tris-buffered saline
    Tris Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tris-buffered+saline/triton+x+100/10__22175_slash_mmb__17646-66-56-66
    Average 90 stars, based on 1 article reviews
    tris-buffered saline - by Bioz Stars, 2026-09
    90/100 stars

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    SDS Page:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Membrane:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Semi-Dry Trans-Blot:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Saline:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Sequencing:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Sterility:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Incubation:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Binding Assay:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Virus:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Recombinant:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Modification:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Blocking Assay:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Software:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Fluorescence:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.

    Microscopy:

    Article Title: The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
    Article Snippet: Monoclonal antibody (MAb) against CF diluted in 0.1% bovine serum albumin-PBS–0.05 Tween 20 was then added (Sigma Aldrich, MO, USA), and the membrane was incubated overnight in a humid chamber and washed with PBS–0.05% Tween. (Sigma Aldrich, MO, USA) Thereafter, a goat anti-mouse immunoglobulin G horseradish peroxidase conjugate solution was added to the membrane and incubated for 2 h in a humid chamber; the bound MAb was detected by the addition of 4-chloro-1-naphthol chromogen (Sigma Aldrich, MO, USA) in Tris-buffered saline (Sigma Aldrich, MO, USA) and H 2 O 2 (Sigma Aldrich, MO, USA).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for histology and FRET studies were prepared by mixing 10 μg of antigen with a filled glycan hole at residues N241 and N289 and 100 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Title Pending 17646
    Article Snippet: The proteins were separated using a 10% SDS-PAGE gel loaded with 40 μg of protein per sample, transferred to a 0.45 μm Nitrocellulose membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), and blocked for 1 h at room temperature with 2% bovine serum albumin (BSA, Sigma Aldrich®) in 1x Tris-Buffered Saline (TBS1x; 50mM Tris-HCL, pH 7.5; 150mM NaCl; Sigma Aldrich®).

    Article Title: Vaccines combining slow delivery and follicle targeting of antigens increase germinal center B cell clonal diversity and clonal expansion
    Article Snippet: Immunizations for sequencing studies were prepared by mixing 5 μg of antigen with a glycan hole at residues N241 and N289 and 50 μg of alum in 100 μL sterile tris-buffered saline (TBS, Sigma Aldrich) per mouse.

    Article Title: Sex Differences Affect the NRF2 Signaling Pathway in the Early Phase of Liver Steatosis: A High-Fat-Diet-Fed Rat Model Supplemented with Liquid Fructose
    Article Snippet: To prevent non-specific antibody binding, the membrane was incubated for 1 h at room temperature in a solution of 5% ( w / v ) low-fat milk (Sigma-Aldrich) and 1% ( w / v ) bovine serum albumin (Sigma-Aldrich) in Tris-buffered saline (137 mM NaCl, 20 mM Tris·HCl, pH 7.6) with 0.1% Tween 20 (Sigma-Aldrich) (TBS-T).

    Article Title: ADAR1 Restricts Poliovirus Infection through dual disruption of translation initiation and protein coding
    Article Snippet: Membranes were blocked with 4% molecular biology grade BSA (Millipore Sigma) in tris-buffered saline supplemented with 0.1% Tween-20 (Millipore Sigma, TBST) for 1 h at RT then probed with primary antibodies for 2 h at RT.

    Article Title: Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models
    Article Snippet: Tris-buffered saline , EMD Millipore , Cat#524750.

    Article Title: Activation of the l -fucose utilization cluster in Campylobacter jejuni induces proteomic changes and enhances Caco-2 cell invasion and fibronectin binding
    Article Snippet: Flat-bottom 96-well plates (Costar, Corning, NY, USA) were coated with a 1-mg/mL solution of Fn in 0.05 M Tris-buffered saline at pH 7.5 (Sigma, Munich, Germany) and incubated overnight at 4 °C.



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